Biol. Pharm. Bull., 30(5),935-940, May 2007

Regular Articles

The Anti-thrombotic Active Constituents from Centella asiatica

Toshiko SATAKE,^a,c Kohei KAMIYA,^*,a,c Yin AN,^a Tomomi OISHI (nee TAKA)^B, and Junichiro YAMAMOTO^b,c

^a Faculty of Pharmaceutical Sciences, Kobe Gakuin University; ^b Faculty of Nutrition, Kobe Gakuin University; and ^c High Technology Research Center, Kobe Gakuin University; Nishi-ku, Kobe 651-2180, Japan. * To whom correspondence should be addressed. e-mail: kamiya@pharm.kobegakuin.ac.jp

The in vitro effects of a methanol extract from the aerial parts of Centella asiatica on shear-induced platelet activation and coagulation were assessed after oral administration to rats, by subjecting non-anticoagulated blood to haemostatometry. 3,5-Di-O-caffeoyl quinic acid, 1,5-di-O-caffeoyl quinic acid, 3,4-di-O-caffeoyl quinic acid, 4,5-di-O-caffeoyl quinic acid, and chlorogenic acid, together with asiaticoside, kaempferol, quercetine, kaempferol-3-O-β-D-glucoside and quercetin-3-O-β-D-glucoside were all isolated from the methanol extract. Amongst these, only 3,5-di-O-caffeoylquinic acid showed significant inhibition of shear-induced platelet activation and dynamic coagulation. The reactive curve of the inhibitory effect on the platelet reaction and the dynamic coagulation showed a bell-shape.

Key words Centella asiatica; anti-thrombotic activity; 3,5-di-O-caffeoylquinic acid; shear stress; non-anticoagulated blood; haemostatometry