Biol. Pharm. Bull., 29(7),1487-1489, July 2006

Notes

Rapid Multiplexing and Simultaneous Detection of Human Spermatogenetic Failure with a 12 Lane Microchip Electrophoresis System

Mohammad JABASINI,^*,a,b Ashraf Abdulazim EWIS,^c Maged FOUAD,^a Fuquan DANG,^c Guichen PING,^a Toshikatsu SHINKA,^d Yutaka NAKAHORI,^d Noritada KAJI,^a Manabu TOKESHI,^a and Yoshinobu BABA^a,c

^a Department of Applied Chemistry, Graduate School of Engineering, Nagoya University; Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan: ^b Toray Industries, Inc., New Frontiers Research Laboratories; 1111 Tebiro, Kamakura, Kanagawa 248-8555, Japan: ^c Nanobio Research Team, Health Technology Research Center, National Institute of Advanced Industrial Science and Technology; 2217-14 Hayashi-cho, Takamatsu 761-0395, Japan: and ^d Department of Human Genetics and Public Health, School of Medicine, The University of Tokushima; Tokushima 770-8505, Japan. * To whom correspondence should be addressed. e-mail: jabasini1@yahoo.com

For the amplification and ultrafast separation of the genetic markers and DNA sequences that are related to human male infertility, a multiplex PCR for amplifying three DNA sequence-tagged sites (STS) located on the human Y chromosome with possible roles in the spermatogenesis process has been designed and applied followed by separation on a microchip. First, the optimum T_m degree for the three DNA markers was optimized and determined experimentally, and the three DNA STS were amplified. These three DNA markers were then separated on a 12-lane microchip electrophoresis system, which can analyze the DNA markers on 12 channels simultaneously. The combination of these two technologies, multiplex PCR and microchip electrophoresis, allows the analysis of 36 DNA markers (12×3) within only 180 s.

Key words spermatogenic failure; multiplex PCR; 12-lane multichannel microchip